Medicinal Chemistry - I Practical Lab Manual : B. Pharm SY IV-Sem

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Welcome to the Medicinal Chemistry – I Practical Lab Manual, a comprehensive guide designed to enhance your understanding of medicinal chemistry through real-world laboratory experiments. This book bridges theoretical concepts and practical skills, focusing on drug design, synthesis, purification, and analysis of pharmaceutical compounds. With clearly written experimental procedures, modern techniques, safety protocols, and ethical practices, this manual prepares students for careers in pharmaceutical sciences, chemical research, and drug discovery. Ideal for academic and industry training, this lab manual supports learning and innovation in medicinal chemistry laboratories and pharmaceutical education.

INDEX

ANNEXURES

A. Planning of experiment
B. Safety in chemistry laboratory
C. Heating and cooling of reaction mixture
D. Thin layer chromatography
E. Recrystallization
F. Aqueous and Non-Aqueous acid base titration

EXPERIMENTS

I) Preparation of drugs/ intermediates

1. Synthesis of 1,3-Pyrazole from Phenylhydrazine
2. Synthesis of 1, 3-oxazole from Hippuric acid
3. Synthesis of Benzimidazole from o-phenylenediamine
4. Synthesis of Benzotriazole from o-phenylenediamine
5. Synthesis of 2,3-Diphenylquinoxaline from o phenylenediamine
6. Synthesis of Benzocaine from p-amino benzoic acid
7. Synthesis of Phenytoin from Benzil
8. Synthesis of Phenothiazine from Diphenyl amine
9. Synthesis of Barbiturate from Urea

II) ASSAY OF DRUGS

1. Assay of Chlorpromazine Hydrochloride
2. Assay of Phenobarbitone
3. Assay of Atropine Sulphate
4. Assay of Ibuprofen
5. Assay of Aspirin.
6. Assay of Furosemide

III) DETERMINATION OF PARTITION COEFFICIENT

1. Partition coefficient of Paracetamol
2. Partition coefficient of Ibuprofen

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Medicinal Chemistry 1

Prabhakar - Prabhakar Publication

Medicinal Chemistry - I Practical Lab Manual : B. Pharm SY IV-Sem

INDEX

ANNEXURES

A. Planning of experiment B. Safety in chemistry laboratory C. Heating and cooling of reaction mixture D. Thin layer chromatography E. Recrystallization F. Aqueous and Non-Aqueous acid base titration

EXPERIMENTS

I) Preparation of drugs/ intermediates

II) ASSAY OF DRUGS

1. Assay of Chlorpromazine Hydrochloride 2. Assay of Phenobarbitone 3. Assay of Atropine Sulphate 4. Assay of Ibuprofen 5. Assay of Aspirin. 6. Assay of Furosemide

III) DETERMINATION OF PARTITION COEFFICIENT

1. Partition coefficient of Paracetamol 2. Partition coefficient of Ibuprofen

Medicinal Chemistry 1

Practical (BP406P)

As per PCI Regulations SECOND YEAR B. PHARM Semester IV

1. Dr. Yogita Ozarde

M. Pharm, Ph.D.

Assistant Professor

Department of Pharmaceutical Science,

School of Health Sciences and Technology,

Dr. Vishwanath Karad MIT World Peace University,

Pune, India

2. Dr. Ranjit Gadhave

M. Pharm, Ph.D.

Associate Professor

Department of Pharmaceutical Science,

School of Health Sciences and Technology,

Dr. Vishwanath Karad MIT World Peace University,

Pune, India

Medicinal Chemistry - 1 Practical (BP406P)

Authors: Dr. Yogita Ozarde, Dr. Ranjit Gadhave

ISBN 978-93-6342-228-5

Copyright Author 2025

First Editon: August 2025

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A. Planning of experiment
B. Safety in chemistry laboratory
C. Heating and cooling of reaction mixture
D. Thin layer chromatography
E. Recrystallization
F. Aqueous and Non-Aqueous acid base titration

1. Assay of Chlorpromazine Hydrochloride
2. Assay of Phenobarbitone
3. Assay of Atropine Sulphate
4. Assay of Ibuprofen
5. Assay of Aspirin.
6. Assay of Furosemide

1. Partition coefficient of Paracetamol
2. Partition coefficient of Ibuprofen